Biochem 630 | Biochemistry

630. Cellular Signal Transduction Mechanisms Fall 2006
Also Pharmacology 630 & Zoology 630 3 cr. Provides a comprehensive introduction for advanced undergraduates and graduate students to the essential elements of cellular transduction mechanisms that allow signaling from the cell surface to the nucleus. Emphasis is on receptors, second messengers, protein kinase cascades, and the regulation of gene transcription. Prerequisites: Introductory Biochemistry (Biochemistry 501, or 507 and 508) and Cell Biology (Biocore 303 or Zoology 570 or Pathology 750) or consent of instructor Organization and logistics Course requirements: Two short papers due Nov 1 & Dec 13; 3 exams by website/email Oct. 14, Nov. 11, and Dec. 16. Asterisks correspond to material for each exam.	Lectures MWF 2:25-3:15 PM; B1118 Biochemistry
	Instructor T. Martin A. Ruoho R. Anderson E. Bresnick S. Miyamoto P. Keely	Email: tfmartin@facstaff.wisc.edu aeruoho@facstaff.wisc.edu raanders@facstaff.wisc.edu ehbresni@facstaff.wisc.edu smiyamot@facstaff.wisc.edu pjkeely@facstaff.wisc.edu
	Teaching Assistant
	E. Kielar	kielar@wisc.edu
Syllabus Each major heading will be covered, in the order indicated. In some cases, a subset of the topics listed under the major heading will be covered.

Date		Speaker	Lectures
Date		Speaker	#	Title	PPT file	PDF file	Movie	Papers
September	6	TM	-	Greetings and Course mechanics
	8	TM	1	Overview of signaling mechanisms: the big picture
	11	TM	2	How are signaling peptides and their receptors synthesized?
	13	TM	3	Proteolysis is required to mature peptides and surface receptors
	15	TM	4	Proteins are destined for constitutive or regulated secretion
	18	AR	5	Basic concept of receptor
	20	AR	6	Basic concept of receptor
	22	AR	7	G protein coupled receptors (GPCRs)
	25	AR	8	G protein coupled receptors (GPCRs)
	27	AR	9	Heterotrimeric G proteins
	29	AR	10	Receptor regulation of adenylyl cyclase
October	2	TM	11	How do GPCRs get turned off after activation?
	4	TM	12	Why does endocytosis of GPCRs occur?
	6	TM	13	Phospholipase C is an important effector for GPCRs
	9	TM	14	How is calcium mobilized as a messenger?
	11	TM	15	How does calcium regulate cell function? (e.g. via calmodulin)
	13	TM	16*	Additional effectors mediate signaling by calcium
	14	Exam 1
	16	RA	17	Cyclic AMP-dependent protein kinase
	18	RA	18	Protein kinase structure and regulation
	20	RA	19	Growth factors, their receptors and cellular functions
	23	RA	20	Growth factor receptor regulation
	25	RA	21	Signaling pathways activated by growth factors
	27	RA	22	Receptor protein tyrosine kinase stimulation of Ras-mediated pathways
	30	RA	23	Activation of PI 3-kinase signaling
November	1	RA	24	Ras signaling PAPER 1 DUE
	3	PK	25	Small G proteins: who, what, where
	6	PK	26	Regulation of small G proteins: GEFs and GAPs
	8	PK	27*	Signaling networks involving RhoGTPases
	10	EB	28	Targeting chromatin organization via cell signaling
	11	Exam 2
	13	EB	29	Targeting the transcriptional apparatus via cell signaling
	15	EB	30	Diverse signaling pathways control transcription
	17	EB	31	Transcriptional control via cAMP signaling
	20	EB	32	Nuclear receptors: the ligand-activated transcription factor paradigm
	22	EB	33	Nuclear receptors: role of protein-protein interactions in signaling
	24	Thanksgiving Break
	27	EB	34	Nuclear receptors: role of multiprotein coactivator/co-repressor complexes in signaling No Problem Set
	29	EB	35	Nuclear receptors: role of multiprotein coactivator/co-repressor complexes in signaling
December	1	SM	36	Death signaling-1: why death signaling is interesting
	4	SM	37	Death signaling-2: cell stress and mitochondria
	6	SM	38	Death signaling-3: death ligand and death receptor
	8	SM	39	NF-kB signaling-1: why NF-kB signaling is interesting
	11	SM	40	NF-kB signaling-2: diverse inputs but a common pathway?
	13	SM	41	NF-kB signaling-3: ubiquitin regulating kinase activation?
	15	SM	42*	Direct pathways to the nucleus: JAK/STAT PAPER 2 DUE
	16	Exam 3

630. Cellular Signal Transduction Mechanisms